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Although chrysene is most clearly associated with human cancer, it has been shown that it is mutagenic in several in vitro mammalian cell systems. Additionally, studies in which chrysene is used in combination with other chemicals has shown that it can have a synergistic effect in mutagenic response. Additionally, the Drosophila melanogaster gene mutant vos has been used to demonstrate this synergism. For these reasons, in US chemical companies, chrysene is considered a Group C carcinogen by OSHA.

Thus, the main purpose of this study is to determine which chemistries are present in the environment that can influence the DNA and, by extrapolation, the human genome in terms of mutation as well as the ability of engineered cells to withstand mutation.

For humans, ingestion of semen from an exposed species by a given species, is the likely pathway of exposure. Ingested semen from animals treated with antiandrogenic and/or estrogens are less likely to induce / retard androgenization. In addition, such treatment will make the gonad to be less sensitive to the effects of estrogen.

There is increasing evidence that EDCs can seriously interfere with maternal, embryonic and fetal development by altering endocrine functions. Several human, animal and laboratory studies have demonstrated major effects of exposure to EDCs on ovarian steroidogenesis and on the development of the placenta and fetus.

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