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To address the dynamics of PCB metabolism and how these changes relate to enantioselective atropisomeric behavior, mice were administered (+/-)-PCB 84, a racemic mixture, and (-)-PCB 84, an enantiomeric form of PCB 84. Both enantiomers are metabolized in a time-dependent manner. The distribution of (-)- and (+)-PCB 84 enantiomers to various tissues and biological fluids is dependent on the administered dose. The (-)-enantiomer was detected in liver, kidney, brain, and milk samples at 1.5 days post-injection. The (+)-enantiomer was detected in the same tissues at 3 days post-injection. A shift in the enantiomeric distribution of PCB 84 and its metabolites as time elapsed was observed. The (-)-enantiomer was preferentially distributed to most tissues, whereas the (+)-enantiomer was preferentially distributed to the liver and kidney. This study suggests that the chiral form of PCB 84 is responsible for the differential distribution of (-)- and (+)-enantiomers in the body. The results presented herein provide a new insight into the understanding of the processes governing the distribution of (-)- and (+)-enantiomers of PCBs.

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